Sucrose responses of IN1 interneurons in fed flies were significantly smaller and lacked persistent activity. Sucrose ingestion caused a rapid and persistent increase in IN1 interneuron activity in fasted flies that decreased proportionally in response to subsequent feeding bouts. We identify 12 cholinergic local interneurons (IN1) necessary for this behavior. Flies showed discrete, temporally precise ingestion that was regulated by hunger state and sucrose concentration.
To study the dynamics of ingestion in the vinegar fly Drosophila melanogaster, we developed Expresso, an automated feeding assay that measures individual meal-bouts with high temporal resolution at nanoliter scale. Ingestion is a highly regulated behavior that integrates taste and hunger cues to balance food intake with metabolic needs.
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